This job posting is anticipated to close on Mar 19 2025. We may however extend this time period, in which case the posting will remain available on www.careers.jnj.com to accept additional applications.
Description
At Johnson & Johnson, we believe health is everything. Our strength in healthcare innovation empowers us to build a world where complex diseases are prevented, treated, and cured, where treatments are smarter and less invasive, and solutions are personal. Through our expertise in Innovative Medicine and MedTech, we are uniquely positioned to innovate across the full spectrum of healthcare solutions today to deliver the breakthroughs of tomorrow, and profoundly impact health for humanity. Learn more at https://www.jnj.com
Job Function:
Discovery & Pre-Clinical/Clinical DevelopmentJob Sub Function:
Biological ResearchJob Category:
Scientific/TechnologyAll Job Posting Locations:
Spring House, Pennsylvania, United States of AmericaJob Description:
Our expertise in Innovative Medicine is informed and inspired by patients, whose insights fuel our science-based advancements. Visionaries like you work on teams that save lives by developing the medicines of tomorrow.
Our Oncology team is focused on the elimination of cancer by discovering new pathways and modalities to finding treatments and cures. We lead where medicine is going and need innovators with an unwavering commitment to results.
Join us in developing treatments, finding cures, and pioneering the path from lab to life while championing patients every step of the way. Learn more at https://www.jnj.com/innovative-medicine
An external pre-identified candidate for consideration has been identified. However, all applications will be considered.
We are searching for a Senior Scientist within Immuno-Oncology, Discovery Oncology Flow Cytometry Core Facility to be in Spring House, PA and have the following responsibilities:
Proactively support Oncology Discovery and Translational Research by providing flow cytometry expertise working within a core facility.
Maintain 15-20 flow cytometers in a multifaceted industry setting. Monitor all calibration results to ensure optimal performance and maintain appropriate instrument configurations. Create/update SOPs for core instrumentation and staining protocols.
Perform all preventative maintenance activities for instruments and computers, including weekly/monthly maintenance procedures, scheduling service appointments, database back-up, quarterly data transfer, collaboration with IT for computer upgrades, and maintaining network and data share accessibility.
Aid users in technical troubleshooting to minimize instrument downtime. Resolve common issues independently, communicate issues to users and update reservation calendars as required.
Process tissue (blood, lymph nodes, tumor, prostate, lung, muscle, spleen, and thymus) to single cell suspension for analysis of immune, tumor, and/or stromal cells by Flow cytometry or other biological methods to support Oncology efforts
Stain and characterize both primary immune cell populations and cell lines by multiparameter (8-40+ colors) flow cytometry, including intracellular and cytokine staining and cell stimulation techniques.
Perform FACS (cell sorting) as a scheduled service of the core facility. Responsibilities include appointment consultation, instrumentation setup and full operation of the cell sorters including technical troubleshooting. Provide data reports to the end user in a timely manner.
Train all new users on flow core protocols and procedures including basic start-up, experimental setup, compensation/unmixing and shutdown procedures. Lead small group hands-on training sessions on instrumentation.
Support all groups with advanced spectral assay/panel design, unmixing set-up, and data analysis using FlowJo and OMIQ platforms applying high dimensional data reduction and visualization techniques.
Maintain detailed and organized experimental records and stay compliant on all relevant internal and external training requirements.
Strong leadership and mentoring abilities, with a passion for developing talent and encouraging a culture of scientific excellence.
Communicate research findings and recommendations effectively through written reports, presentations, and scientific publications.
Remain current on the latest flow cytometry trends, assays, and technology and reagents to share across the department. Identify emerging trends and opportunities in the field and provide strategic recommendations to senior management.
Ability to travel to attend specific trainings / conferences as needed up to 10% of time.
Qualifications:
Ph.D. in a relevant scientific subject area (e.g., Chemistry, Cell Biology, Pharmacology, Biochemistry) with a minimum of 2 years of industry or academic experience within a flow cytometry core facility. Alternatively, Masters w/ 4-6 years of relevant experience or Bachelor’s w/ 7-9 years of relevant experience.
At least 1 year experience with full spectral high parameter multi-color flow cytometry (25+ colors) including panel design best practices, spectral unmixing, fluorochrome compatibility, and data QC is required.
Experience with technical troubleshooting on conventional and spectral instrumentation and corresponding software from vendors including BD is required. Cytek, or Sartorious, or SONY experience is highly desired.
Demonstrated proficiency with high parameter (25+ color) panel design, assay optimization, qualification and assay transfer procedures for the evaluation of clinical trial samples is required.
Independent operation of FACS (cell sorting) instrumentation on BD platforms and basic troubleshooting is required.
Demonstrated strong leadership and mentoring abilities to train new users on the theory and practical application of flow cytometry is required.
Experience in design and interpretation of cell-based assays/experiments to interrogate questions in immunology and oncology is highly desired.
Experience with aseptic technique, cell culture and basic immunogenicity assays is preferred.
Experience with in vivo models of tumor biology, tissue processing, and subsequent flow cytometry workflows is preferred
Excellent and timely communication/collaboration skills are required.
Johnson & Johnson is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, age, national origin, disability, protected veteran status or other characteristics protected by federal, state or local law. We actively seek qualified candidates who are protected veterans and individuals with disabilities as defined under VEVRAA and Section 503 of the Rehabilitation Act.
Johnson and Johnson is committed to providing an interview process that is inclusive of our applicants’ needs. If you are an individual with a disability and would like to request an accommodation, please email the Employee Health Support Center (ra-employeehealthsup@its.jnj.com) or contact AskGS to be directed to your accommodation resource.
